Emergence of a large pore subpopulation during electroporation pulses
Kyle C. Smith, Reuben S. Son, Thiruvallur R. Gowrishankar, James C. Weaver Bioelectrochem. 100:3-10. 2014.
Electroporation increases ionic and molecular transport through cell
membranes by creating transient aqueous pores. These pores cannot be
directly observed experimentally, but cell system modeling with dynamic
electroporation predicts pore populations that produce cellular
responses consistent with experiments. We show a cell system model's
response that illustrates the life cycle of a pore population in
response to a widely used 1 kV/cm, 100 us trapezoidal pulse. Rapid
pore creation occurs early in the pulse, followed by the gradual
emergence of a subpopulation of large pores reaching ~30 nm radius.
After the pulse, pores rapidly contract to form a single thermally
broadened distribution of small pores (~1 nm radius) that slowly decays.
We also show the response of the same model to pulses of 100 ns to
1 ms duration, each with an applied field strength adjusted such
that a total of 10,000±100 pores are created. As pulse duration is
increased, the pore size distributions vary dramatically and a
distinct subpopulation of large pores emerges for pulses of microsecond
and longer duration. This subpopulation of transient large pores
is relevant to understanding rapid transport of macromolecules into
and out of cells during a pulse.