Charged microbeads are not transported across the human stratum corneum in vitro by short high-voltage pulses
Chen T; Langer R; Weaver JC;
Bioelectrochemistry and Bioenergetics 48: (1) 181-192 FEB 1999
There have been several reports of particle transport due to high-voltage
pulsing of human skin. Here, several different short, high-voltage pulsing
protocols were used in vitro to study the possible transport of highly
charged, fluorescent polystyrene particles (14 nm to 2.1 mu m in diameter;
surface charges of -4.05 x 10(3) e to -2.77 X 10(7) e) across the skin.
Two different methods were used to trap and measure particles on the
other side of the skin. The first used a polycarbonate membrane to trap
the particles, determining the amount of transport by enumeration under
a fluorescence microscope. The second used spectrofluorimetry to measure
the amount of particles transported. After pulsing, particles were found
in randomly distributed clusters on the surface of the skin. No detectable
transport across the stratum corneum for any size particle was observed.