Transdermal transport of DNA antisense oligonucleotides by electroporation.
Zewert TE; Pliquett UF; Langer R; Weaver JC Biochem Biophys Res Commun (9Y8), 1995 Jul 17; 212 (2): 286-92
Fluorescein-labeled antisense oligodeoxynucleotides (ODNs)
corresponding to the promoters of the protooncogene c-myb
(24-mer) and the oncogene c-myc (15-mer) were transported
through the human skin in vitro by electroporation. Fluxes
of 6.4 +/- 2.1 pM/cm2* hr and 11.5 +/- 3.5 pM/cm2* hr,
respectively, were achieved during exponential pulsing [tau
pulse = 1.1ms, transdermal voltage (Uskin) = 80V] every five
seconds. The flux for Uskin < 70V was undetectable,
rapidly increased at 80V, but plateaued at higher values.
Fluorescence imaging demonstrated that transport of the ODNs
is concentrated in localized transport regions (LTRs)1
approximately 30 microns in diameter.