Electroporation: high frequency of occurrence of a transient
high-permeability state in erythrocytes and intact yeast.
Weaver JC; Harrison GI; Bliss JG; Mourant JR; Powell KT FEBS Lett (EUH), 1988 Feb 29; 229 (1): 30-4
We present the first determinations of population
distributions of macromolecule uptake due to
electroporation, the percentage of cells which participate
and, for the yeast, the subpopulation of cells whose
membranes exhibit significant recovery following
macromolecule uptake. Flow cytometry is used to measure the
uptake of a first test molecule (green fluorescence, FITC-
dextran; 70 kDa) and also, for the yeast, the subsequent
uptake of a second, much smaller, test molecule (red
fluorescence, propidium iodide; 660 Da), which provides a
measure of membrane recovery. A dramatic 20% (erythrocytes)
to 75% (intact Schizosaccharomyces pombe) of cells can take
up the first test molecule within 5 min of a pulse.